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Enzyme Nanocapsules as Intracellular Scarvagers for Reactive Oxygen Species

  • Author(s): Li, Shuoran
  • Advisor(s): Segura, Tatiana
  • Lu, Yunfeng
  • et al.
Abstract

We use superoxide and catalase synergy nanocapsule system to reduce the cytotoxicity caused by superoxide radicals, a typical reactive oxygen species (ROS). Enzymes are encapsulated inside positive shells via APS/TEMED initiated polymerization. The diameter and charge of the non-degradable nanocapsules are suitable for intracellular delivery and clear endocytosis has been observed via fluorescence microscope. Under induced radical environment, higher cell viability is observed when cells are incubated with both nanocapsules at the ratio of nSOD/nCatalase=5 compared with only nSOD or nCatalase system. We believe that the use of this synergy delivery providing a sound progress for eliminating ROS in vitro via synergy protein therapy. What's more, this bi-enzyme nanocapsule system can also be seen as solid foundation for multi-enzyme nanocapsule system to achieve other complex therapeutic applications.

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