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Open Access Publications from the University of California

Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells.

  • Author(s): Huang, Xiao
  • Hu, Qirui
  • Lai, Yifan
  • Morales, Demosthenes P
  • Clegg, Dennis O
  • Reich, Norbert O
  • et al.
Abstract

A new method of spatially controlled gene regulation in 3D-cultured human embryonic stem cells is developed using hollow gold nanoshells (HGNs) and near-infrared (NIR) light. Targeted cell(s) are discriminated from neighboring cell(s) by focusing NIR light emitted from a two-photon microscope. Irradiation of cells that have internalized HGNs releases surface attached siRNAs and leads to concomitant gene downregulation.

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