Skip to main content
eScholarship
Open Access Publications from the University of California

UC Santa Cruz

UC Santa Cruz Previously Published Works bannerUC Santa Cruz

RNA-binding protein CPEB1 remodels host and viral RNA landscapes.

  • Author(s): Batra, Ranjan;
  • Stark, Thomas J;
  • Clark, Elizabeth;
  • Belzile, Jean-Philippe;
  • Wheeler, Emily C;
  • Yee, Brian A;
  • Huang, Hui;
  • Gelboin-Burkhart, Chelsea;
  • Huelga, Stephanie C;
  • Aigner, Stefan;
  • Roberts, Brett T;
  • Bos, Tomas J;
  • Sathe, Shashank;
  • Donohue, John Paul;
  • Rigo, Frank;
  • Ares, Manuel;
  • Spector, Deborah H;
  • Yeo, Gene W
  • et al.

Published Web Location

https://doi.org/10.1038/nsmb.3310
Abstract

Host and virus interactions occurring at the post-transcriptional level are critical for infection but remain poorly understood. Here, we performed comprehensive transcriptome-wide analyses revealing that human cytomegalovirus (HCMV) infection results in widespread alternative splicing (AS), shortening of 3' untranslated regions (3' UTRs) and lengthening of poly(A)-tails in host gene transcripts. We found that the host RNA-binding protein CPEB1 was highly induced after infection, and ectopic expression of CPEB1 in noninfected cells recapitulated infection-related post-transcriptional changes. CPEB1 was also required for poly(A)-tail lengthening of viral RNAs important for productive infection. Strikingly, depletion of CPEB1 reversed infection-related cytopathology and post-transcriptional changes, and decreased productive HCMV titers. Host RNA processing was also altered in herpes simplex virus-2 (HSV-2)-infected cells, thereby indicating that this phenomenon might be a common occurrence during herpesvirus infections. We anticipate that our work may serve as a starting point for therapeutic targeting of host RNA-binding proteins in herpesvirus infections.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View