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A role for aurora A kinase in timely nuclear envelope breakdown

  • Author(s): Portier, Nathan Charles
  • et al.

The Aurora kinases are a family of mitotic kinases that are responsible for a variety of functions within the cell. Depletion of the centrosome-associated family member, Aurora A, results several phenotypes, including defects in centrosome maturation, centrosome separation, robust aster and spindle nucleation, and loss of cell polarity. We have used depletions of the Aurora A homolog, AIR-1, in C. elegans early embryonic divisions to discover a novel role for the kinase in timely nuclear envelope breakdown (NEBD). Here, we describe the generation of an assay used to determine the extent of chromosome condensation using fluorescence time-lapse images as input. This assay allowed us to determine that depletion of AIR-1 does not affect the onset or rate of chromosome condensation, rather a specific delay in NEBD is seen. AIR-1 depletion also affects the progression of nuclear envelope permeabilization once it has begun, with a coordinate slowing of lamin and nuclear pore component protein loss. Further, protein depletions that result in a centrosome maturation defect demonstrate an intermediate NEBD delay with respect to air-1(RNAi) embryos

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