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The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

  • Author(s): Rautengarten, C
  • Ebert, B
  • Liu, L
  • Stonebloom, S
  • Smith-Moritz, AM
  • Pauly, M
  • Orellana, A
  • Scheller, HV
  • Heazlewood, JL
  • et al.
Abstract

© 2016 The Author(s). Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

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