UC Santa Cruz

Currently, the entire globe is affected by the COVID-19 pandemic caused by the novel coronavirus, SARS-CoV-2(SCV2). The ability to detect, monitor and assess the spread of the virus is imperative to controlling the effects of the pandemic. In order to meet the need for global testing, several methods have been developed. As a complement to individual testing, wastewater-based epidemiology represents a low-cost way to estimate the prevalence of the virus in a community. This information can be used to influence public policy regarding viral mitigation measures. This thesis documents our efforts at implementing wastewater testing in Santa Cruz County over the past 18 months. We sampled from the Watsonville Wastewater Treatment Plant on a weekly basis and quantified the presence of SCV2 using reverse transcriptase digital PCR normalized to Pepper Mild Mottle Virus (PMMV). Our data showed a large discrepancy between reported case counts and SCV2 in wastewater. This data also revealed seasonal variation in PMMV, potentially hindering it as a reliable normalizer. In addition to our sewage work, we performed masked-based sampling for Pacific Elementary School and documented a probable positive. This work demonstrates the potential of sewage sampling for SARS-CoV-2 and some of the current unmet challenges in both sewage sampling and pandemic response as a whole.