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Actin filament assembly by bacterial factors VopL/F: Which end is up?
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https://doi.org/10.1083/jcb.201702165Abstract
Competing models have been proposed for actin filament nucleation by the bacterial proteins VopL/F. In this issue, Burke et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201608104) use direct observation to demonstrate that VopL/F bind the barbed and pointed ends of actin filaments but only nucleate new filaments from the pointed end.
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