4Pi fluorescence detection and 3D particle localization with a single objective
- Author(s): Huang, Bo
- et al.
Published Web Locationhttp://europepmc.org/articles/PMC3796685?pdf=render
Coherent detection through two opposing objectives (4Pi configuration) improves the precision of three-dimensional (3D) singlemolecule localization substantially along the axial direction, but suffers from instrument complexity and maintenance difficulty.