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4Pi fluorescence detection and 3D particle localization with a single objective

Published Web Location

http://europepmc.org/articles/PMC3796685?pdf=render
No data is associated with this publication.
Abstract

Coherent detection through two opposing objectives (4Pi configuration) improves the precision of three-dimensional (3D) singlemolecule localization substantially along the axial direction, but suffers from instrument complexity and maintenance difficulty.

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