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Function-based identification of mammalian enhancers using site-specific integration
- Dickel, Diane E;
- Zhu, Yiwen;
- Nord, Alex S;
- Wylie, John N;
- Akiyama, Jennifer A;
- Afzal, Veena;
- Plajzer-Frick, Ingrid;
- Kirkpatrick, Aileen;
- Göttgens, Berthold;
- Bruneau, Benoit G;
- Visel, Axel;
- Pennacchio, Len A
Published Web Location
https://doi.org/10.1038/nmeth.2886Abstract
Putative enhancer elements of a genomic region of interest are cloned in front of a reporter gene, integrated in a single site in mouse embryonic stem cells, and screened for enhancer activity using flow cytometry and high-throughput sequencing.
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