Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil.
- Ribeiro, Geovani de Oliveira;
- Monteiro, Fred Julio Costa;
- Rego, Marlisson Octavio da S;
- Ribeiro, Edcelha Soares D'Athaide;
- Castro, Daniela Funayama de;
- Caseiro, Marcos Montani;
- Souza Marinho, Robson Dos Santos;
- Komninakis, Shirley Vasconcelos;
- Witkin, Steven S;
- Deng, Xutao;
- Delwart, Eric;
- Sabino, Ester Cerdeira;
- da Costa, Antonio Charlys;
- Leal, Élcio
- et al.
Published Web Locationhttps://doi.org/10.3390/v11020147
Advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected by the virome analysis of fecal samples from migratory birds in Australia. We now report the detection of RNA-dependent RNA polymerase sequences of HRLV 7 in pools of Aedes aegypti and Culex quinquefasciatus mosquitoes species from the Brazilian Amazon forest. Phylogenetic inferences indicated that all HRLV 7 strains fall within the same independent clade. In addition, HRLV 7 shared a close ancestral lineage with the Dinovernavirus genus of the Reoviridae family. Our findings indicate that HRLV 7 is present in two species of mosquitoes.