Bioluminescence Imaging of Angiogenesis in a Murine Orthotopic Pancreatic Cancer Model
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Bioluminescence Imaging of Angiogenesis in a Murine Orthotopic Pancreatic Cancer Model

  • Author(s): Angst, Eliane
  • Chen, Monica
  • Mojadidi, Michelle
  • Hines, O. Joe
  • Reber, Howard A.
  • Eibl, Guido
  • et al.
Abstract

Angiogenesis is essential for physiological processes as well as for carcinogenesis. New approaches to cancer therapy include targeting angiogenesis. One target is VEGF-A and its receptor VEGFR2. In this study, we sought to investigate pancreatic cancer angiogenesis in a genetically modified VEGFR2-luc-KI mouse. Live in vivo bioluminescence imaging of angiogenesis was performed continuously until sacrifice in subcutaneous tumors as well as in orthotopically transplanted tumors. Tumor tissue was immunostained for CD-31 and VEGFR2. Peritumoral angiogenesis measured by light emission was detected beginning at week 3 following subcutaneous injection. In the orthotopic model, light emission began at day 4, which likely corresponds to wound healing, and continued throughout the experimental period during tumor growth. Peritumoral CD-31 vessel- and VEGFR2-staining were positive. The VEGFR2-luc-KI mouse is a valuable tool to demonstrate tumor angiogenesis and seems to be suitable to evaluate anti-angiogenic approaches in pancreatic cancer.

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