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Live Cell Imaging of Endogenous mRNA Using RNA-Based Fluorescence “Turn-On” Probe

Published Web Location

https://doi.org/10.1021/acschembio.6b00586
Abstract

Messenger RNA (mRNA) plays a critical role in cellular growth and development. However, there have been limited methods available to visualize endogenous mRNA in living cells with ease. We have designed RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences. These probes have been demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging.

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