Homoserine and Threonine Peptide Assembly
- Author(s): Pirrung, Michael C
- Bakas, Nicole A
- et al.
Published Web Locationhttps://doi.org/10.1055/s-0036-1589123
Drawing on our recent success with reagent-less peptide-bond formation through serine-based assembly reactions in organic solvent, their range has been expanded to threonine and homoserine (an aspartic acid precursor) in the N-terminal peptide. Amino acid scope available at the assembly C-terminus includes bulky residues not amenable to classical ligation methods, such as cysteine-based NCL in aqueous media. The method was used to assemble a snakebite-toxin-neutralizing peptide from opossums.