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The crystal structure of a bacterial Sufu-like protein defines a novel group of bacterial proteins that are similar to the N-terminal domain of human Sufu.

  • Author(s): Das, Debanu;
  • Finn, Robert D;
  • Abdubek, Polat;
  • Astakhova, Tamara;
  • Axelrod, Herbert L;
  • Bakolitsa, Constantina;
  • Cai, Xiaohui;
  • Carlton, Dennis;
  • Chen, Connie;
  • Chiu, Hsiu-Ju;
  • Chiu, Michelle;
  • Clayton, Thomas;
  • Deller, Marc C;
  • Duan, Lian;
  • Ellrott, Kyle;
  • Farr, Carol L;
  • Feuerhelm, Julie;
  • Grant, Joanna C;
  • Grzechnik, Anna;
  • Han, Gye Won;
  • Jaroszewski, Lukasz;
  • Jin, Kevin K;
  • Klock, Heath E;
  • Knuth, Mark W;
  • Kozbial, Piotr;
  • Krishna, S Sri;
  • Kumar, Abhinav;
  • Lam, Winnie W;
  • Marciano, David;
  • Miller, Mitchell D;
  • Morse, Andrew T;
  • Nigoghossian, Edward;
  • Nopakun, Amanda;
  • Okach, Linda;
  • Puckett, Christina;
  • Reyes, Ron;
  • Tien, Henry J;
  • Trame, Christine B;
  • van den Bedem, Henry;
  • Weekes, Dana;
  • Wooten, Tiffany;
  • Xu, Qingping;
  • Yeh, Andrew;
  • Zhou, Jiadong;
  • Hodgson, Keith O;
  • Wooley, John;
  • Elsliger, Marc-André;
  • Deacon, Ashley M;
  • Godzik, Adam;
  • Lesley, Scott A;
  • Wilson, Ian A
  • et al.

Published Web Location

https://doi.org/10.1002/pro.497
Abstract

Sufu (Suppressor of Fused), a two-domain protein, plays a critical role in regulating Hedgehog signaling and is conserved from flies to humans. A few bacterial Sufu-like proteins have previously been identified based on sequence similarity to the N-terminal domain of eukaryotic Sufu proteins, but none have been structurally or biochemically characterized and their function in bacteria is unknown. We have determined the crystal structure of a more distantly related Sufu-like homolog, NGO1391 from Neisseria gonorrhoeae, at 1.4 Å resolution, which provides the first biophysical characterization of a bacterial Sufu-like protein. The structure revealed a striking similarity to the N-terminal domain of human Sufu (r.m.s.d. of 2.6 Å over 93% of the NGO1391 protein), despite an extremely low sequence identity of ∼15%. Subsequent sequence analysis revealed that NGO1391 defines a new subset of smaller, Sufu-like proteins that are present in ∼200 bacterial species and has resulted in expansion of the SUFU (PF05076) family in Pfam.

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