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The crystal structure of a bacterial Sufu-like protein defines a novel group of bacterial proteins that are similar to the N-terminal domain of human Sufu.

  • Author(s): Das, Debanu
  • Finn, Robert D
  • Abdubek, Polat
  • Astakhova, Tamara
  • Axelrod, Herbert L
  • Bakolitsa, Constantina
  • Cai, Xiaohui
  • Carlton, Dennis
  • Chen, Connie
  • Chiu, Hsiu-Ju
  • Chiu, Michelle
  • Clayton, Thomas
  • Deller, Marc C
  • Duan, Lian
  • Ellrott, Kyle
  • Farr, Carol L
  • Feuerhelm, Julie
  • Grant, Joanna C
  • Grzechnik, Anna
  • Han, Gye Won
  • Jaroszewski, Lukasz
  • Jin, Kevin K
  • Klock, Heath E
  • Knuth, Mark W
  • Kozbial, Piotr
  • Krishna, S Sri
  • Kumar, Abhinav
  • Lam, Winnie W
  • Marciano, David
  • Miller, Mitchell D
  • Morse, Andrew T
  • Nigoghossian, Edward
  • Nopakun, Amanda
  • Okach, Linda
  • Puckett, Christina
  • Reyes, Ron
  • Tien, Henry J
  • Trame, Christine B
  • van den Bedem, Henry
  • Weekes, Dana
  • Wooten, Tiffany
  • Xu, Qingping
  • Yeh, Andrew
  • Zhou, Jiadong
  • Hodgson, Keith O
  • Wooley, John
  • Elsliger, Marc-André
  • Deacon, Ashley M
  • Godzik, Adam
  • Lesley, Scott A
  • Wilson, Ian A
  • et al.

Published Web Location

https://doi.org/10.1002/pro.497
Abstract

Sufu (Suppressor of Fused), a two-domain protein, plays a critical role in regulating Hedgehog signaling and is conserved from flies to humans. A few bacterial Sufu-like proteins have previously been identified based on sequence similarity to the N-terminal domain of eukaryotic Sufu proteins, but none have been structurally or biochemically characterized and their function in bacteria is unknown. We have determined the crystal structure of a more distantly related Sufu-like homolog, NGO1391 from Neisseria gonorrhoeae, at 1.4 Å resolution, which provides the first biophysical characterization of a bacterial Sufu-like protein. The structure revealed a striking similarity to the N-terminal domain of human Sufu (r.m.s.d. of 2.6 Å over 93% of the NGO1391 protein), despite an extremely low sequence identity of ∼15%. Subsequent sequence analysis revealed that NGO1391 defines a new subset of smaller, Sufu-like proteins that are present in ∼200 bacterial species and has resulted in expansion of the SUFU (PF05076) family in Pfam.

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