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Dual roles of FBXL3 in the mammalian circadian feedback loops are important for period determination and robustness of the clock.

  • Author(s): Shi, Guangsen
  • Xing, Lijuan
  • Liu, Zhiwei
  • Qu, Zhipeng
  • Wu, Xi
  • Dong, Zhen
  • Wang, Xiaohan
  • Gao, Xiang
  • Huang, Moli
  • Yan, Jie
  • Yang, Ling
  • Liu, Yi
  • Ptácek, Louis J
  • Xu, Ying
  • et al.
Abstract

The mammalian circadian clock is composed of interlocking feedback loops. Cryptochrome is a central component in the core negative feedback loop, whereas Rev-Erbα, a member of the nuclear receptor family, is an essential component of the interlocking loop. To understand the roles of different clock genes, we conducted a genetic interaction screen by generating single- and double-mutant mice. We found that the deletion of Rev-erbα in F-box/leucine rich-repeat protein (Fbxl3)-deficient mice rescued its long-circadian period phenotype, and our results further revealed that FBXL3 regulates Rev-Erb/retinoic acid receptor-related orphan receptor-binding element (RRE)-mediated transcription by inactivating the Rev-Erbα:histone deacetylase 3 corepressor complex. By analyzing the Fbxl3 and Cryptochrome 1 double-mutant mice, we found that FBXL3 also regulates the amplitudes of E-box-driven gene expression. These two separate roles of FBXL3 in circadian feedback loops provide a mechanism that contributes to the period determination and robustness of the clock.

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