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Effects of Sample Treatments on Genome Recovery via Single-Cell Genomics

  • Author(s): Woyke, Tanja
  • Clingenpeel, Scott
  • Schwientek, Patrick
  • Hugenholtz, Philip
  • et al.
Abstract

Single-cell genomics is a powerful tool for accessing genetic information from uncultivated microorganisms. Methods of handling samples before single-cell genomic amplification may affect the quality of the genomes obtained. Using three bacterial strains we show that compared to cryopreservation, lower quality single-cell genomes are recovered when the sample is preserved in ethanol or if the sample undergoes fluorescence in situ hybridization, while sample preservation in paraformaldehyde renders it completely unsuitable for sequencing

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