Skip to main content
Open Access Publications from the University of California

Effects of Sample Treatments on Genome Recovery via Single-Cell Genomics


Single-cell genomics is a powerful tool for accessing genetic information from uncultivated microorganisms. Methods of handling samples before single-cell genomic amplification may affect the quality of the genomes obtained. Using three bacterial strains we show that compared to cryopreservation, lower quality single-cell genomes are recovered when the sample is preserved in ethanol or if the sample undergoes fluorescence in situ hybridization, while sample preservation in paraformaldehyde renders it completely unsuitable for sequencing

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View