Skip to main content
eScholarship
Open Access Publications from the University of California

Translatome Regulation in Neuronal Injury and Axon Regrowth.

  • Author(s): Rozenbaum, Meir
  • Rajman, Marek
  • Rishal, Ida
  • Koppel, Indrek
  • Koley, Sandip
  • Medzihradszky, Katalin F
  • Oses-Prieto, Juan A
  • Kawaguchi, Riki
  • Amieux, Paul S
  • Burlingame, Alma L
  • Coppola, Giovanni
  • Fainzilber, Mike
  • et al.
Abstract

Transcriptional events leading to outgrowth of neuronal axons have been intensively studied, but the role of translational regulation in this process is not well understood. Here, we use translatome analyses by ribosome pull-down and protein synthesis characterization by metabolic isotopic labeling to study nerve injury and axon outgrowth proteomes in rodent dorsal root ganglia (DRGs) and sensory neurons. We identify over 1600 gene products that are primarily translationally regulated in DRG neurons after nerve injury, many of which contain a 5'UTR cytosine-enriched regulator of translation (CERT) motif, implicating the translation initiation factor Eif4e in the injury response. We further identified approximately 200 proteins that undergo robust de novo synthesis in the initial stages of axon growth. ApoE is one of the highly synthesized proteins in neurons, and its receptor binding inhibition or knockout affects axon outgrowth. These findings provide a resource for future analyses of the role of translational regulation in neuronal injury responses and axon extension.

Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.

Main Content
Current View