The Rolling Circle Amplification to Concatemeric Consensus (R2C2) protocol was developed to increase the accuracy of Oxford Nanopore Technologies (ONT) long reads for transcriptomic analysis. Originally designed to transform cDNA input into long reads containing multiple copies of individual molecules, this protocol has a wide range of applications using various types of input. I demonstrate the versatility of R2C2 with three general types of input: Illumina libraries, 10X single cell RNA libraries, and plasmids. I developed the PLNK (Processing Live Sequencing experiments) software to monitor ONT MinION runs of Illumina R2C2 libraries to analyze the read quality, library content and coverage over target regions. I used 10X single cell RNA libraries as input for R2C2 and analyzed them with Mandalorion to produce cell type specific transcriptomes. Finally, I shortened the R2C2 protocol to allow for plasmid input and created Chopper, a software pipeline which produces accurate assemblies of full length plasmids.