Recently, the integration of a wide range of nanocomponents has been investigated for building patterned or layered structures on the macroscopic and mesoscopic scale. In order to break through the issues of current devices and develop diverse and reliable applications from nanobiomaterials to nanoelectronics, it is necessary to fabricate nano-devices and systems using nanocomponents. However, traditional fabrication methods have required the modification of these nanomaterials, and need new approaches for the advancement of development for diverse and reliable applications. In this dissertation, two novel methods are demonstrated for pragmatic nano devices and systems : Controlling extrinsic nanoparticle alignment by electrical field deposition, and intrinsic DNA binding through photolithography. First, the use of nanoparticles' electrophoretic deposition (EPD) onto porous biodegradable polymer allows the production of transparent flexible carbon nanotube network films with mechanical strength. Most importantly, after solvent treatment, the opaque substrate changed to transparent with conductivity. Thus, we produced flexible transparent films. Another unique aspect of this process is that after solvent treatment, the substrate can be implanted onto newspapers or cloth. Second, we demonstrate a DNA double write process that represents, which allows DNA to be used as a unique material for UV patterning, with subsequent selfassembly via the hybridization of complementary DNA sequences. This novel method allows true synergy for combining top-down photolithography with bottom-up selfassembly. In addition, we have been able to demonstrate both first- and second-level patterning, including target sequence detection and streptavidin / biotin binding with the DNA double write process