In this study, two separate experiments were conducted with an F10 Brassica napus recombinant inbred (RI) population containing 124 lines derived from a cross between P-efficient cv. 97081 and P-inefficient cv. 97009 by a single seed descent method. One was two-year field trials under the conditions of high P and low P with three replicates, which was used to investigate seed yield and analyze minerals (P, Ca, Mg, Fe, Zn, Mn, and Cu) concentration in seeds with ICP-AES. Another was two paper culture experiments with high P and low P treatments and three replicates, which were used to investigate root morphology, including total root length, root surface area, root volume and root average diameter with WinRhizo analysis system. Based on the construction of genetic linkage map, WinQTLCart software was employed to identify the quantitative trait loci (QTL) for seed minerals concentration and root morphology by whole genome QTL analysis. Totally, 49 QTL for mineral nutrients in seeds and 61 QTL for root morphology were detected, which explained the phenotypic variance from 8.59% to 36.48%. Most of these QTL corresponded specifically to one of the two P conditions, and intervals of some QTL for different traits overlap each other. These results revealed that genetic factors control seed mineral concentration in plants and multigenes involving ion transport are required to regulate mineral balance in plants under conditions of diverse nutrient stress.
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