Whole glove testing for a metalworking fluid (MWF) in the field was performed for the first time. Green latex gloves used in a machine shop were exposed for 20 min to MWF. The permeated amount (1.0 ± 0.5 μg/cm2) was higher than the threshold (0.25 μg/cm2) for the ASTM F739-99a closed-loop normalized breakthrough time.