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Positron emission tomography probe demonstrates a striking concentration of ribose salvage in the liver.

  • Author(s): Clark, Peter M
  • Flores, Graciela
  • Evdokimov, Nikolai M
  • McCracken, Melissa N
  • Chai, Timothy
  • Nair-Gill, Evan
  • O'Mahony, Fiona
  • Beaven, Simon W
  • Faull, Kym F
  • Phelps, Michael E
  • Jung, Michael E
  • Witte, Owen N
  • et al.

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PET is a powerful technique for quantifying and visualizing biochemical pathways in vivo. Here, we develop and validate a novel PET probe, [(18)F]-2-deoxy-2-fluoroarabinose ([(18)F]DFA), for in vivo imaging of ribose salvage. DFA mimics ribose in vivo and accumulates in cells following phosphorylation by ribokinase and further metabolism by transketolase. We use [(18)F]DFA to show that ribose preferentially accumulates in the liver, suggesting a striking tissue specificity for ribose metabolism. We demonstrate that solute carrier family 2, member 2 (also known as GLUT2), a glucose transporter expressed in the liver, is one ribose transporter, but we do not know if others exist. [(18)F]DFA accumulation is attenuated in several mouse models of metabolic syndrome, suggesting an association between ribose salvage and glucose and lipid metabolism. These results describe a tool for studying ribose salvage and suggest that plasma ribose is preferentially metabolized in the liver.

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