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Positron emission tomography probe demonstrates a striking concentration of ribose salvage in the liver

  • Author(s): Clark, PM
  • Flores, G
  • Evdokimov, NM
  • McCracken, MN
  • Chai, T
  • Nair-Gill, E
  • O'Mahony, F
  • Beaven, SW
  • Faull, KF
  • Phelps, ME
  • Jung, ME
  • Witte, ON
  • et al.

Published Web Location

http://www.ncbi.nlm.nih.gov/pubmed/24982199
No data is associated with this publication.
Abstract

PET is a powerful technique for quantifying and visualizing biochemical pathways in vivo. Here, we develop and validate a novel PET probe, [18F]-2-deoxy-2-fluoroarabinose ([18F]DFA), for in vivo imaging of ribose salvage. DFA mimics ribose in vivo and accumulates in cells following phosphorylation by ribokinase and further metabolism by transketolase. We use [18F]DFA to show that ribose preferentially accumulates in the liver, suggesting a striking tissue specificity for ribose metabolism. We demonstrate that solute carrier family 2, member 2 (also known as GLUT2), a glucose transporter expressed in the liver, is one ribose transporter, but we do not know if others exist. [18F]DFA accumulation is attenuated in several mouse models of metabolic syndrome, suggesting an association between ribose salvage and glucose and lipid metabolism. These results describe a tool for studying ribose salvage and suggest that plasma ribose is preferentially metabolized in the liver.

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