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Transcriptional analysis of the multiple Sry genes and developmental program at the onset of testis differentiation in the rat
Published Web Location
https://link.springer.com/article/10.1186/s13293-020-00305-8No data is associated with this publication.
Abstract
Background
The commonly used laboratory rat, Rattus norvegicus, is unique in having multiple Sry gene copies found on the Y chromosome, with different copies encoding amino acid variations that influence the resulting protein function. It is not clear which Sry genes are expressed at the onset of testis differentiation or how their expression correlates with that of other genes in testis-determination pathways.Methods
Here, two independent E11-E14 developmental RNAseq datasets show that multiple Sry genes are expressed at E12-E13.Results
The identified copies expressed during testis initiation include Sry4A, Sry1, and Sry3C, which are conserved in every strain of Rattus norvegicus with genomes sequenced to date.Conclusions
This work represents a first step in defining the complex environment of rat testis differentiation that can open the door for generating sex reversal model systems using embryo manipulation techniques that have been available in the mouse but not the rat.Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.