Non-Invasive Quantification of Cell Density in 3D Gels by MRI
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Non-Invasive Quantification of Cell Density in 3D Gels by MRI

  • Author(s): Archer, Brian
  • Ueberrueck, Till
  • Mack, Julia
  • Youssef, Khalid
  • Jarenwattananon, Nanette N
  • Rall, Deniz
  • Wypysek, Denis
  • Wiese, Martin
  • Bluemich, Bernhard
  • Wessling, Matthias
  • Iruela-Arispe, Luisa
  • Bouchard, Louis
  • et al.

Published Web Location

https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=8412441&tag=1
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Abstract

— Objective: For tissue engineering, there is a need for quantitative methods to map cell density inside 3D bioreactors to assess tissue growth over time. The current cell mapping methods in 2D cultures are based on optical microscopy. However, optical methods fail in 3D due to increased opacity of the tissue. We present an approach for measuring the density of cells embedded in a hydrogel to generate quantitative maps of cell density in a living, 3D tissue culture sample. Methods: Quantification of cell density was obtained by calibrating the 1H T2, magnetization transfer (MT) and diffusion-weighted nuclear magnetic resonance (NMR) signals to samples of known cell density. Maps of cell density were generated by weighting NMR images by these parameters post-calibration. Results: The highest sensitivity weighting arose from MT experiments, which yielded a limit of detection (LOD) of 2.5 × 108 cells/mL/√ Hz in a 400 MHz (9.4 T) magnet. Conclusion: This mapping technique provides a noninvasive means of visualizing cell growth within optically opaque bioreactors. Significance: We anticipate that such readouts of tissue culture growth will provide valuable feedback for controlled cell growth in bioreactors.

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