We first introduce the formulation of 2D periodic dyadic Green's function to account for all the field contributions required to thoroughly describe the physics of a 2D array of nanospheres on top of a multilayered substrate. Then, we analyze substrate effects onto complex modes and optical properties of 2D arrays of linear trimers of plasmonic nanospheres and show that Fano resonant features appear for oblique TM-polarized plane wave incidence illumination. These features are attributed to the forced excitation of free modes supported by the array, here computed via modal analysis. We observe strengthened Fano features due to the presence of the multilayered substrate. © 2013 IEEE.