Unburned yucca (Yucca) quids with wild tobacco (Nicotiana) contents have been preserved within Antelope Cave in northwestern Arizona. Although the cave was visited during the Archaic, Southern Paiute, and Euro-American periods, material culture remains and radiocarbon dates indicate the heaviest use by the Virgin Anasazi (A.D. 1-1000). Quids are wads of fiber twisted or knotted into a ball for insertion into the mouth. Ten of the quids examined were clearly made from the fibers of Yucca plants, based on 6-7 base pairs identified via analysis of DNA sequences near the trnL gene of chloroplastic DNA. Twenty-seven of thirty quids examined were wrapped around a range of wild tobacco (Nicotiana) plant parts (e.g., capsule, seed, calyx, pedicel, main stem, leaf). Quids have been interpreted as serving various needs (food, ceremonial/ritual, other). The inclusion of tobacco and the diverse recovery contexts suggest the Antelope Cave quids provided occupants with a personal stimulant experience.