A novel virus-like sequence from grapevine was identified by Illumina sequencing. The genomic organization was most similar to that of members of the genus Fabavirus Polyproteins RNA-1 and RNA-2 of the virus tentatively named grapevine fabavirus (GFabV) shared 34 to 23% sequence identities with Broad bean wilt virus 2 (BBWV2), respectively. GFabV was successfully graft transmitted to Vitis vinifera cv. Cabernet Franc.