We report the development and characterization of nine microsatellite markers from the Tridacna crocea genome. In a wild population of 20 individuals from Ulugan Bay in the Republic of the Philippines, we observed an average of 9 alleles per locus and expected heterozygosity ranging from 0.41 to 0.92. Cross-species priming was tested in three additional Tridacna species with moderate success. These markers will be used in studies of fine-scale population genetic structure and gene flow in T. crocea. The markers may also be useful in a forensic context by identifying source populations of giant clams collected illegally for the live aquarium trade, while protecting legal exports from aquaculture facilities.