I examined the effects of road mortality on a population of western painted turtles (Chrysemys picta belli) in west-central Montana; these turtles make up the majority of road mortalities in a section of highway that bisects the Ninepipes National Wildlife Refuge. The objective of my barrier fencing experiment was to determine whether turtles were able to breach fencing designed to direct turtles towards crossing structures and thereby keep them off the road. I constructed 45.7-cm-high turtle enclosures out of 2- by 5-cm fencing with and without 10- or 15-cm-high flashing attached at the top. Turtles were placed in the enclosures, and behavior was observed for one hour. Of 124 turtles, only four (3.2%) were able to climb to the flashing. No turtles climbed over the flashing within the time allowed. In enclosures without flashing, two (3.8%) were able to breach the fencing. The results of this experiment will help in the design of appropriate barriers to keep turtles off the road and direct them towards crossing structures.