- Ingargiola, Antonino;
- Peronio, Pietro;
- Lerner, Eitan;
- Gulinatti, Angelo;
- Rech, Ivan;
- Ghioni, Massimo;
- Weiss, Shimon;
- Michalet, Xavier
- Editor(s): Enderlein, Jörg;
- Gregor, Ingo;
- Gryczynski, Zygmunt K;
- Erdmann, Rainer;
- Koberling, Felix
Single-molecule spectroscopy on freely-diffusing molecules allows detecting conformational changes of biomolecules without perturbation from surface immobilization. Resolving fluorescence lifetimes increases the sensitivity in detecting conformational changes and overcomes artifacts common in intensity-based measurements. Common to all freely-diffusing techniques, however, are the long acquisition times. We report a time-resolved multispot system employing a 16-channel SPAD array and TCSPC electronics, which overcomes the throughput issue. Excitation is obtained by shaping a 532 nm pulsed laser into a line, matching the linear SPAD array geometry. We show that the line-excitation is a robust and cost-effective approach to implement multispot systems based on linear detector arrays.