In Arabidopsis thaliana the majority of the fruit comprises an ovary with three primary tissue regions; the valve, the valve margin and the replum. Interestingly, there exists active cell proliferation in the replum domain, which leads to the formation of specialized structures located inside of the ovary, including the ovules. This feature makes the replum tissue to be considered one of the "quasi-meristem" structures present in the Arabidopsis thaliana fruit. A set of regulatory genes has been identified to control different aspects of replum development and growth, including the replum identity genes RPL and BP. However, keeping in mind the meristem-like features that the replum has we wanted to explore whether additional meristem-related genes are also impinging upon replum formation. We have uncovered an additional player participating in replum morphopgenesis, which, interestingly, is post-transcriptionally regulated by small RNAs. A comprehensive and detailed analysis of this novel factor in the context of replum development, as well as the importance of its regulation by small RNA in this developmental process are the main focuses of this work

The PacBio^® HiFi sequencing method yields highly accurate long-read sequencing datasets with read lengths averaging 10-25 kb and accuracies greater than 99.5%. These accurate long reads can be used to improve results for complex applications such as single nucleotide and structural variant detection, genome assembly, assembly of difficult polyploid or highly repetitive genomes, and assembly of metagenomes. Currently, there is a need for sample data sets to both evaluate the benefits of these long accurate reads as well as for development of bioinformatic tools including genome assemblers, variant callers, and haplotyping algorithms. We present deep coverage HiFi datasets for five complex samples including the two inbred model genomes Mus musculus and Zea mays, as well as two complex genomes, octoploid Fragaria × ananassa and the diploid anuran Rana muscosa. Additionally, we release sequence data from a mock metagenome community. The datasets reported here can be used without restriction to develop new algorithms and explore complex genome structure and evolution. Data were generated on the PacBio Sequel II System.