- Chung, Brian T;
- Chen, Hsin-Yu;
- Gordon, Jeremy;
- Mammoli, Daniele;
- Sriram, Renuka;
- Autry, Adam W;
- Le Page, Lydia M;
- Chaumeil, Myriam M;
- Shin, Peter;
- Slater, James;
- Tan, Chou T;
- Suszczynski, Chris;
- Chang, Susan;
- Li, Yan;
- Bok, Robert A;
- Ronen, Sabrina M;
- Larson, Peder EZ;
- Kurhanewicz, John;
- Vigneron, Daniel B
We developed methods for the preparation of hyperpolarized (HP) sterile [2-13C]pyruvate to test its feasibility in first-ever human NMR studies following FDA-IND & IRB approval. Spectral results using this MR stable-isotope imaging approach demonstrated the feasibility of investigating human cerebral energy metabolism by measuring the dynamic conversion of HP [2-13C]pyruvate to [2-13C]lactate and [5-13C]glutamate in the brain of four healthy volunteers. Metabolite kinetics, signal-to-noise (SNR) and area-under-curve (AUC) ratios, and calculated [2-13C]pyruvate to [2-13C]lactate conversion rates (kPL) were measured and showed similar but not identical inter-subject values. The kPL measurements were equivalent with prior human HP [1-13C]pyruvate measurements.