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Decoding the mechanisms of cancer and stem cell immortality

  • Author(s): Chiba, Kunitoshi
  • Advisor(s): Hockemeyer, Dirk
  • et al.
Abstract

Telomeres are the repetitive sequences at the ends of linear chromosomes. The

key functions of telomeres are to protect the cells from losing genomic information and to

prevent chromosome ends from being repaired by the double strand break repair

machinery. To counteract loss of telomeric DNA, cells can express a reverse

transcriptase, telomerase, that synthesizes telomeric repeats de novo. In humans,

telomerase activity is mostly restricted to germ and stem cells, so the telomeres of most

somatic cells progressively shorten with each cell division. Once telomeres become

critically short, they are recognized as sites of DNA damage and cells cease to proliferate.

By this mechanism, telomere shortening functions as a tumor suppression mechanism.

TERT, the protein component of telomerase, becomes silenced once stem cells

differentiate. However, in 90% of cancer cells, TERT is transcriptionally re-activated.

Thus, telomerase regulation is crucial for our understanding of telomere length regulation

in stem cell maintenance and tumorigenesis. To understand how telomerase acts on

telomeres, I attempted to endogenously tag telomerase. To do this I inserted epitope tags

at the endogenous TERT locus in hESCs using genome editing. However, I found that all

the tested tags cause defects in telomere maintenance, which was previously not

appreciated in experiments using exogenous overexpression.

Recently, point mutations in the TERT promoter were identified as the most

frequent non-coding mutations in cancer. To elucidate the role of TERT promoter

mutations (TPMs) in tumorigenesis, I genetically engineered these TPMs into human

embryonic stem cells (hESCs) using genome editing. Using the resulting isogenic hESC

lines, I demonstrated that TPMs lead to a failure of TERT silencing upon differentiation

from stem into somatic cells. To understand role of TPMs in tumorigenesis, I monitored

long-term telomere maintenance and proliferation in human fibroblasts engineered to

carry TPMs. I found that TPMs immortalize cells but do not prevent telomere shortening

and telomere fusions. In vitro, around the time when telomere fusions occurred, TERT

expression was gradually increased. Thus, TPMs are required, but not sufficient, for

cancer cell immortality and contribute to tumorigenesis in two steps. First, TPMs expand

proliferation capacity of a cell by elongating only the shortest telomeres but do not

prevent overall telomere shortening. In the second step, TPMs fuel tumorigenesis by not

fully suppressing genomic instability. In order for cells to immortalize they need to

upregulate TERT during this second step.

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