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Effects of Collagenase type II on Vitreous Humor, an in-situ Rheological Study.

  • Author(s): Rangchian, Aysan;
  • Francone, Anibal;
  • Farajzadeh, Matthew;
  • Hosseini, Helia;
  • Connelly, Kelly;
  • Hubschman, Jean Pierre;
  • Kavehpour, Pirouz
  • et al.

Published Web Location

https://doi.org/10.1115/1.4043358
Abstract

The purpose of this study is to quantify the impact of enzyme activity on the vitreous humor structure over time to understand the mechanical characteristics of the vitreous humor gel. Changes in the mechanical behavior of the vitreous occur to many reasons including aging, which may lead to many vitreoretinal diseases. The degeneration of the vitreous has been studied; however, in-situ experimental procedures to validate the existing hypotheses are limited. We examined thirty-eight porcine eyes using in-situ rheological creep tests to measure the mechanical properties of the vitreous humor of the eyes prior to, 1 and 24 hours after the intravitreal injection. Eyes in one group were injected with collagenase type II solution and eyes in the control group were injected with Phosphate Buffered Saline solution with calcium and magnesium chloride. Prior to the injection, viscosity and creep compliance intercept values between both groups were not statistically different. At 1 hour and 24 hours after the injection, vitreous properties in eyes from the first group showed a statistically significant increase in the J intercept (representing the inverse of elasticity) values compared with the control group. In addition, 1 and 24 hours after the injection, vitreous viscosity was lower in eyes from the first group than in eyes from the control group. These findings are a foundation for future studies on the effectiveness of intravitreal drugs to modify the mechanical properties of the vitreous humor.

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