Skip to main content
Open Access Publications from the University of California


UCLA Previously Published Works bannerUCLA

A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion.

  • Author(s): Mallinson, Sam JB;
  • Machovina, Melodie M;
  • Silveira, Rodrigo L;
  • Garcia-Borràs, Marc;
  • Gallup, Nathan;
  • Johnson, Christopher W;
  • Allen, Mark D;
  • Skaf, Munir S;
  • Crowley, Michael F;
  • Neidle, Ellen L;
  • Houk, Kendall N;
  • Beckham, Gregg T;
  • DuBois, Jennifer L;
  • McGeehan, John E
  • et al.

Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View