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Fluorescent Labeling of RNA and DNA on the Hoogsteen Edge using Sulfinate Chemistry
- Bassi, Tiziano Giorgio
- Advisor(s): Toor, Navtej
Abstract
We have devised a single pot, low-cost method to add azide groups to unmodified nucleic acids without the need for enzymes or chemically modified nucleoside triphosphates. This involves reacting an azide-containing sulfinate salt with thenucleic acid, leading to replacement of C–H bonds on the nucleobase aromatic rings with C–R, where R is the azide-containing linker derived from the original sulfinate salt. With the addition of azide functional groups, the modified nucleicacid can easily be reacted with any alkyne-labeled compound of interest, including fluorescent dyes as shown in this work. This methodology enables the fluorescent labeling of a wide variety of nucleic acids, including natively folded RNAs, under mild conditions with minimal effects upon biochemical function and ribozyme catalysis. To demonstrate this, we show that a pair of labeled complementary ssDNA oligonucleotides (oligos) can hybridize to form dsDNA, even when labeled with multiple fluorophores per oligo. In addition, we also demonstrate that two different group II introns can splice when prelabeled internally with fluorophores, using our method. Broadly, this demonstrates that sulfinate modification of RNA is compatible with ribozyme function and Watson–Crick pairing, while preserving the labile backbone.
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