UCLA

snoRNAs are small non-coding RNAs which guide post-transcriptional modification of cellular RNAs. Here we present work investigating the processing of these critical RNAs. Many snoRNAs are encoded within the introns of coding genes. We show that in the absence of splicing, highly stable aberrant transcripts are generated. During the course of this work, we employed long-read RNA sequencing (ONT). ONT seq is an exciting new tool for studying RNA isoforms but visualization tools are scarce. Here we present our long-read sequencing visualization tool, NanoBlot and suggest future development directions. Finally, The TRAMP complex is a trimeric protein complex with semi-modular protein subunits. It is responsible for degradation and processing of a number of RNAs including excised introns and snoRNAs. We present work investigating the substrate specificity of 2 TRAMP confirmations.