Skip to main content
eScholarship
Open Access Publications from the University of California
Refine Search

UCLA

UCLA Previously Published Works bannerUCLA

Aligning the μs-ALEX Setup.

Published Web Location

https://doi.org/10.1101/pdb.prot086421
Abstract

To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G(550-620)R(650-750).

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.


Top