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Aligning the μs-ALEX Setup.

  • Author(s): Kapanidis, Achillefs;
  • Majumdar, Devdoot;
  • Heilemann, Mike;
  • Nir, Eyal;
  • Weiss, Shimon
  • et al.
Abstract

To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G(550-620)R(650-750).

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