Skip to main content
eScholarship
Open Access Publications from the University of California

Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus

  • Author(s): Lawrenson, K
  • Kar, S
  • McCue, K
  • Kuchenbaeker, K
  • Michailidou, K
  • Tyrer, J
  • Beesley, J
  • Ramus, SJ
  • Li, Q
  • Delgado, MK
  • Lee, JM
  • Aittomäki, K
  • Andrulis, IL
  • Anton-Culver, H
  • Arndt, V
  • Arun, BK
  • Arver, B
  • Bandera, EV
  • Barile, M
  • Barkardottir, RB
  • Barrowdale, D
  • Beckmann, MW
  • Benitez, J
  • Berchuck, A
  • Bisogna, M
  • Bjorge, L
  • Blomqvist, C
  • Blot, W
  • Bogdanova, N
  • Bojesen, A
  • Bojesen, SE
  • Bolla, MK
  • Bonanni, B
  • Børresen-Dale, AL
  • Brauch, H
  • Brennan, P
  • Brenner, H
  • Bruinsma, F
  • Brunet, J
  • Buhari, SA
  • Burwinkel, B
  • Butzow, R
  • Buys, SS
  • Cai, Q
  • Caldes, T
  • Campbell, I
  • Canniotto, R
  • Chang-Claude, J
  • Chiquette, J
  • Choi, JY
  • Claes, KBM
  • Cook, LS
  • Cox, A
  • Cramer, DW
  • Cross, SS
  • Cybulski, C
  • Czene, K
  • Daly, MB
  • Damiola, F
  • Dansonka-Mieszkowska, A
  • Darabi, H
  • Dennis, J
  • Devilee, P
  • Diez, O
  • Doherty, JA
  • Domchek, SM
  • Dorfling, CM
  • Dörk, T
  • Dumont, M
  • Ehrencrona, H
  • Ejlertsen, B
  • Ellis, S
  • Engel, C
  • Lee, E
  • Evans, DG
  • Fasching, PA
  • Feliubadalo, L
  • Figueroa, J
  • Flesch-Janys, D
  • et al.
Abstract

© 2016 The Author(s). A locus at 19p13 is associated with breast cancer (BC) and ovarian cancer (OC) risk. Here we analyse 438 SNPs in this region in 46,451 BC and 15,438 OC cases, 15,252 BRCA1 mutation carriers and 73,444 controls and identify 13 candidate causal SNPs associated with serous OC (P=9.2 × 10-20), ER-negative BC (P=1.1 × 10-13), BRCA1-associated BC (P=7.7 × 10-16) and triple negative BC (P-diff=2 × 10-5). Genotype-gene expression associations are identified for candidate target genes ANKLE1 (P=2 × 10-3) and ABHD8 (P<2 × 10-3). Chromosome conformation capture identifies interactions between four candidate SNPs and ABHD8, and luciferase assays indicate six risk alleles increased transactivation of the ADHD8 promoter. Targeted deletion of a region containing risk SNP rs56069439 in a putative enhancer induces ANKLE1 downregulation; and mRNA stability assays indicate functional effects for an ANKLE1 3′-UTR SNP. Altogether, these data suggest that multiple SNPs at 19p13 regulate ABHD8 and perhaps ANKLE1 expression, and indicate common mechanisms underlying breast and ovarian cancer risk.

Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.

Main Content
Current View