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Open Access Publications from the University of California

Effects of T1-CCDC90Bon the Mitochondria in Human Mammary Epithelial Cells

  • Author(s): Ravi, Ritesh
  • Advisor(s): Wang, Jean
  • et al.
Abstract

Coiled-Coil Domain Containing 90B (CCDC90B) is a protein of unknown function, which has three functional transcripts according to Ensembl genome browser. In this project, we showed that T1-CCDC90B does, in fact, localize to the mitochondria in MCF-10A cells as there was co-localization via immunofluorescence between CCDC90B protein staining and the activity of the mitochondria. We also showed that when we overproduced T1-CCDC90B protein, there was an increase in COX1 protein in MCF10A cells. An increase in the T1 transcript also caused an increased in the mitochondrial potential per total mitochondrial compartment size and this difference was directly due to a reduction in the size of the mitochondrial compartment. Furthermore, we reversed these effects by stable knockdown of CCDC90B protein in cells and saw an increase in the mitochondrial compartment size.

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