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Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing
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https://doi.org/10.1038/nmeth.1552Abstract
In vivo calcium imaging at multiple depths simultaneously is shown using multifocal two-photon microscopy and spatiotemporal multiplexing. This technique involves scanning the sample with multiple beams in parallel at different axial planes and is applied to monitor neuronal network activity in multiple cortical layers of an anesthetized mouse.
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