UC San Diego

While there has been a great deal of research trying to understand the link between DNA information the regulatory level in the final morphological outcome of an organism, a deep understanding of this relationship remains elusive. Here I attempted a new integrated approach to problem by : 1) comparing DNA sequences taken from different animals that have been diversified during evolution to generate altered function. 2) exploring a novel approach to dissecting cis-regulatory modules (CRMs) using in locus Cas9 genome editing; and 3) developing the mutagenic chain reaction, a novel active genetic element that can be use to easily perform cis-regulatory analysis in organisms with no existing genetic tools