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Spatial frequency domain tomography of protoporphyrin IX fluorescence in preclinical glioma models

  • Author(s): Konecky, Soren D.
  • Owen, Chris M.
  • Rice, Tyler
  • Valdes, Pablo A.
  • Kolste, Kolbein
  • Wilson, Brian C.
  • Leblond, Frederic
  • Roberts, David W.
  • Paulsen, Keith D.
  • Tromberg, Bruce J.
  • et al.
Abstract

Abstract.Multifrequency (0 to 0.3  mm−1), multiwavelength (633, 680, 720, 800, and 820 nm) spatial frequency domain imaging (SFDI) of 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) was used to recover absorption, scattering, and fluorescence properties of glioblastoma multiforme spheroids in tissue-simulating phantoms and in vivo in a mouse model. Three-dimensional tomographic reconstructions of the frequency-dependent remitted light localized the depths of the spheroids within 500 μm, and the total amount of PpIX in the reconstructed images was constant to within 30% when spheroid depth was varied. In vivo tumor-to-normal contrast was greater than ∼1.5 in reduced scattering coefficient for all wavelengths and was ∼1.3 for the tissue concentration of deoxyhemoglobin (ctHb). The study demonstrates the feasibility of SFDI for providing enhanced image guidance during surgical resection of brain tumors.

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