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Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments.

  • Author(s): Isozaki, A
  • Nakagawa, Y
  • Loo, MH
  • Shibata, Y
  • Tanaka, N
  • Setyaningrum, DL
  • Park, J-W
  • Shirasaki, Y
  • Mikami, H
  • Huang, D
  • Tsoi, H
  • Riche, CT
  • Ota, T
  • Miwa, H
  • Kanda, Y
  • Ito, T
  • Yamada, K
  • Iwata, O
  • Suzuki, K
  • Ohnuki, S
  • Ohya, Y
  • Kato, Y
  • Hasunuma, T
  • Matsusaka, S
  • Yamagishi, M
  • Yazawa, M
  • Uemura, S
  • Nagasawa, K
  • Watarai, H
  • Di Carlo, D
  • Goda, K
  • et al.
Abstract

Droplet microfluidics has become a powerful tool in precision medicine, green biotechnology, and cell therapy for single-cell analysis and selection by virtue of its ability to effectively confine cells. However, there remains a fundamental trade-off between droplet volume and sorting throughput, limiting the advantages of droplet microfluidics to small droplets (<10 pl) that are incompatible with long-term maintenance and growth of most cells. We present a sequentially addressable dielectrophoretic array (SADA) sorter to overcome this problem. The SADA sorter uses an on-chip array of electrodes activated and deactivated in a sequence synchronized to the speed and position of a passing target droplet to deliver an accumulated dielectrophoretic force and gently pull it in the direction of sorting in a high-speed flow. We use it to demonstrate large-droplet sorting with ~20-fold higher throughputs than conventional techniques and apply it to long-term single-cell analysis of Saccharomyces cerevisiae based on their growth rate.

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