Stable isotope analyses of biological tissues that grow during different phases of life can be used to trace the geographic location of individuals during different windows of time. We apply this principle and reexamine three prehistoric skeletons excavated in 1964 from archaeological site CA-YOL-117 in Central California. Field evidence suggests they were killed as part of a single violent event. We report new radiocarbon dates and Strontium, Oxygen, Carbon, and Nitrogen isotopic data from first molars, third molars, and bone to examine circumstances surrounding their death. Data suggest two of the three individuals were born, and all three lived their teenage years, near the site they were buried in. However, as adults they lived elsewhere, likely to the north along the Sacramento River. Around AD 1450, upon returning to the territory of their childhood years, they were killed and placed in a mass grave. The data invite a more nuanced interpretation of how we view "local" and "non-local" individuals archaeologically. Overall, the analysis provides an interesting glimpse into the nature of warfare and violence among pre-contact hunter-gatherers of Northern California. © 2013 Elsevier Ltd.

Metallographic, chemical and lead isotopic analyses of copper-based artefacts recovered from the Northwest Quarter in Jerash (ancient Gerasa) in Jordan provide new information on the civic life and material culture from a key urban site in the Roman Empire's eastern provinces. The samples span the city's occupation from its flourishing under Roman rule into the Byzantine and early Islamic periods. We examined 49 copper-based artefacts were examined using reflected light microscopy and micro-X-ray fluorescence. A subset of these artefacts was analysed by electron microprobe spectroscopy for major and minor elements at higher spatial resolution, and by multicollector inductively coupled plasma mass spectrometry for lead isotopes. Results imply that binary bronze dominated the Roman period, (leaded) brass characterised the Byzantine period, while tin-containing alloys were prevalent during the Islamic period. Lead isotopes suggest that during the Roman and Byzantine periods some of the metal in Jerash came from European and/or Mediterranean sources, while copper used during the Islamic period may have been sourced more locally from Timna. The changes in alloy types and lead isotopes suggest that recycling of metals took place in Jerash possibly as early as the Roman period and more frequent from the Byzantine period onwards.

We explore strontium (Sr) isotope analysis as a means to reconstruct ancient migration patterns of individuals at SCL-287, a Middle Period site in southern San Francisco Bay. Comparison of Sr isotopes from rst molars to that of bone suggests that males frequently immigrated to the site, while all females were born at or near the site. This pattern is consistent with a preference for matrilocal post-marital residence patterns. At the same time, analysis of third molars indicates that individuals frequently shifted residence during early teenage years, even those who were born and lived as adults at SCL-287. While adult residence shifts are commonly reported in the ethnography of Central California, matrilocality is not. In this respect, isotopic analyses allow us to generate new hypotheses about ancient migration patterns in prehistory independent of those reported in the ethnographic literature.

Archaeological glass contains information about the movement of goods and ancient economies, yet our understanding of critical aspects of the ancient glass industry is fragmentary. During Roman times, distinct glass types produced in coastal regions of Egypt and the Levant used evaporitic soda (natron) mixed with Nile-derived sands. In the Levant, furnaces for producing colourless Roman glass by addition of manganese have been uncovered, whereas the source of the desirable antimony-decolourised Roman glass remains an enigma. In the Edict of Diocletian, this colourless glass is listed as "Alexandrian" referring to Egypt, but its origin has been ambiguous. Previous studies have found overlapping strontium and neodymium isotope ratios for Levantine and Egyptian glass. Here, we confirm these findings and show for the first time, based on glasses from the ancient city of Gerasa, that hafnium (Hf) isotopes are different in Egyptian and Levantine natron glasses, and that Sb Roman glass is Egyptian. Our work illustrates the value of Hf isotopes in provenancing archaeological glass. We attribute the striking difference in Hf isotopes of Egyptian versus Levantine glasses to sorting of zircons in Nile sediments during longshore drift and aeolian transport along the south-eastern Mediterranean coast leaving behind a less juvenile fraction.

The Nasca culture of the south coast of Peru developed during the first millennium ad and is known internationally for its elaborately decorated polychrome pots. Despite decades of iconographic analysis, little is known about the more technological aspects of Nasca pigment production and application. We present results from a pilot study on iron isotopes as a potential line of inquiry into the differences between red and black pigments in Nasca pigments. As well, we conduct a small firing experiment to examine potential changes in isotope composition. Our analyses show three significant results. First, firing does not change the isotopic ratios of Fe in pigments. Second, red and black pigments show differences in their iron isotope composition, which relate to differences in the minerals used to make the different colors. Third, naturally available hematite samples show greater isotopic variation than pigment samples, suggesting that people selected a limited range of iron oxides to produce pigments. © 2013 Springer-Verlag Berlin Heidelberg.

Background

Methylmercury (MeHg), known for well over a century as a neurotoxin in adults, has more recently been studied for potential detrimental effects during early brain development. While several studies have estimated mercury exposure, they usually rely on either a single biomarker or questionnaire data, each of which has limitations. The goal of this paper was to develop a toxicokinetic model that incorporates both biomarker and questionnaire data to estimate the cumulative exposure to MeHg through seafood consumption using data collected from the Childhood Autism Risks from Genetics and the Environment (CHARGE) study.

Methods

We utilized a previously described discrete-time model that estimates blood MeHg concentration given a piecewise-constant ingestion rate and single-compartment pharmacokinetics. We measured newborn bloodspot Hg concentrations and obtained information pertaining to maternal fish consumption using a questionnaire. Using MeHg concentration estimates from the toxicokinetic model, cumulative MeHg exposure was estimated in children with autism, children with developmental delay, and typically developing children. Median estimated cumulative MeHg was compared among diagnostic groups using the Kruskal-Wallis Test. Multinomial logistic regression models were constructed to assess the association between cumulative MeHg concentration and the risk of autism and developmental delay (vs. typical development).

Results

The estimated average MeHg concentration of for all fish species consumed by mothers was 42 ppb. Median cumulative MeHg over gestation was similar across diagnostic groups (p-values raged from 0.91 to 0.98). After adjusting for potential confounding, we found no association between cumulative MeHg exposure and the risk of autism (OR = 0.95, 95% CI: 0.95, 1.12) or developmental delay (OR = 1.00, 95% CI: 0.89, 1.13).

Conclusions

The toxicokinetic model described in this paper yielded fish MeHg concentration estimates that are consistent with fish species containing lower levels of MeHg. Overall, cumulative MeHg exposure does not appear to detectably elevate the risk of autism or developmental delay. Based on the regression standard error for the association between ASD and TD, we would have reported statistical significance for an adjusted odds ratio of 1.09 or larger. This method can easily be extended to other epidemiologic studies in which there is a biomarker measurement and questionnaire data regarding exposure.

The D” layer at the base of the Earth’s mantle exhibits anomalous seismic properties, which are attributed to heat loss from and chemical interaction with the underlying molten Fe-rich outer core. Here we show that mass transfer due to temperature variations within the D” layer could lead to resolvable fractionation of iron isotopes. We constrain the degree of isotope fractionation by experiments on core-forming Fe alloy liquids at 2100–2300 K and 2 GPa, which demonstrate that heavy Fe isotopes preferentially migrate towards lower temperature and vice versa. We find that this isotope fractionation occurs rapidly due to the high mobility of iron, which reaches 0.013 ± 0.002‰ (2σ) per degree per amu at steady state. Numerical simulations of mantle convection capturing the evolution of a basal thermal boundary layer show that iron isotope fractionation immediately above the core–mantle boundary can reach measurable levels on geologic timescales and that plumes can entrain this fractionated material into the convecting mantle. We suggest that such a process may contribute to the heavy Fe isotope composition of the upper mantle inferred from mantle melts (basalts) and residues (peridotites) relative to chondrites. That being the case, non-traditional stable isotope systems such as Fe may constrain the interactions between the core and mantle.

Analysis of a mass burial of seven males at CA-ALA-554, a prehistoric site in the Amador Valley, CA, was undertaken to determine if the individuals were "locals" or "non-locals," and how they were genetically related to one another.The study includes osteological, genetic (mtDNA), and stable (C, N, O, S) and radiogenic (Sr) isotope analyses of bone and tooth (first and third molars) samples.Isotopes in first molars, third molars, and bone show they spent the majority of their lives living together. They are not locals to the Amador Valley, but were recently living to the east in the San Joaquin Valley, suggesting intergroup warfare as the cause of death. The men were not maternally related, but represent at least four different matrilines. The men also changed residence as a group between age 16 and adult years.Isotope data suggest intergroup warfare accounts for the mass burial. Genetic data suggest the raiding party included sets of unrelated men, perhaps from different households. Generalizing from this case and others like it, we hypothesize that competition over territory was a major factor behind ancient warfare in Central California. We present a testable model of demographic expansion, wherein villages in high-population-density areas frequently fissioned, with groups of individuals moving to lower-population-density areas to establish new villages. This model is consistent with previous models of linguistic expansion.