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An Immunoarray for the Simultaneous Determination of Multiple Triazine Herbicides

  • Author(s): Wortberg, Monika
  • Kreissig, Sabine B.
  • Jones, Geoffrey
  • Rocke, David
  • Hammock, Bruce D.
  • et al.
Abstract

An immunochemical method for simultaneous analysis of cross-reacting analytes is presented. We demonstrate the general principle using triazine herbicides as the model system. The analysis is based on a combination of individual enzyme immunoassays (immunoarray) for triazine herbicides using antibodies with different cross-reactivity patterns towards the selected analytes. The assay signals obtained can be mathematically evaluated to estimate concentrations of each analyte out of a ternary or quaternary mixture. The mathematical model utilizes an extension of the empirical four parameter log-logistic fit. Using mono- and polyclonal antibodies it was possible to quantify the four analytes atrazine, simazine, cyanazine, and prometon in the low to sub-ppb range simultaneously.

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