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The IBEX knowledge-base a community resource enabling adoption and development of immunofluorescence imaging methods
- Yaniv, Ziv;
- Anidi, Ifeanyichukwu U;
- Arakkal, Leanne;
- Arroyo-Mejías, Armando J;
- Beuschel, Rebecca T;
- Börner, Katy;
- Chu, Colin J;
- Clark, Beatrice H;
- Clatworthy, Menna R;
- Colautti, Jake;
- Coscia, Fabian;
- Croteau, Joshua;
- Denha, Saven;
- Dever, Rose;
- Dutra, Walderez O;
- Fritzsche, Sonja;
- Fullam, Spencer;
- Gerner, Michael Y;
- Gola, Anita;
- Gollob, Kenneth J;
- Hernandez, Jonathan M;
- Hor, Jyh Liang;
- Ichise, Hiroshi;
- Jing, Zhixin;
- Jonigk, Danny;
- Kandov, Evelyn;
- Kastenmüller, Wolfgang;
- Koenig, Joshua FE;
- Kothurkar, Aanandita;
- Kortekaas, Rosa K;
- Kreins, Alexandra Y;
- Lamborn, Ian T;
- Lin, Yuri;
- Morais, Katia Luciano Pereira;
- Lunich, Aleksandra;
- Luz, Jean CS;
- MacDonald, Ryan B;
- Makranz, Chen;
- Maltez, Vivien I;
- McDonough, John E;
- Moriarty, Ryan V;
- Ocampo-Godinez, Juan M;
- Olyntho, Vitoria Murakami;
- Oxenius, Annette;
- Padhan, Kartika;
- Remmert, Kirsten;
- Richoz, Nathan;
- Schrom, Edward C;
- Shang, Wanjing;
- Shi, Lihong;
- Shih, Rochelle M;
- Speranza, Emily;
- Stierli, Salome;
- Teichmann, Sarah A;
- Verse, Tibor Z;
- Vierhout, Megan;
- Wachter, Brianna T;
- Wade-Vallance, Adam K;
- Williams, Margaret;
- Zangger, Nathan;
- Germain, Ronald N;
- Radtke, Andrea J
Published Web Location
https://doi.org/10.7554/elife.105737Abstract
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D and 3D immunofluorescence imaging methods. The design of the Knowledge-Base is modeled after efforts in the open-source software community and includes three facets: a development platform (GitHub), static website, and service for data archiving. The Knowledge-Base facilitates the practice of open science throughout the research life cycle by providing validation data for recommended and non-recommended reagents, such as primary and secondary antibodies. In addition to reporting negative data, the Knowledge-Base empowers method adoption and evolution by providing a venue for sharing protocols, videos, datasets, software, and publications. A dedicated discussion forum fosters a sense of community among researchers while addressing questions not covered in published manuscripts. Together, scientists from around the world are advancing scientific discovery at a faster pace, reducing wasted time and effort, and instilling greater confidence in the resulting data.
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