Skip to main content
eScholarship
Open Access Publications from the University of California

Impact of donor age and weaning status on pancreatic exocrine and endocrine tissue maturation in pigs

  • Author(s): Krishnan, R
  • Truong, N
  • Gerges, M
  • Stiewig, M
  • Neel, N
  • Ho-Nguyen, K
  • Kummerfeld, C
  • Alexander, M
  • Spizzo, T
  • Martin, M
  • Foster, CE
  • Monuki, ES
  • Lakey, JRT
  • et al.

Published Web Location

https://doi.org/10.1111/xen.12184
Abstract

© 2015 John Wiley and Sons A/S. Published by John Wiley and Sons Ltd. Background During the process of islet isolation, pancreatic enzymes are activated and released, adversely affecting islet survival and function. We hypothesize that the exocrine component of pancreases harvested from pre-weaned juvenile pigs is immature and hence pancreatic tissue from these donors is protected from injury during isolation and prolonged tissue culture. Methods Biopsy specimens taken from pancreases harvested from neonatal (5-10 days), pre-weaned juvenile (18-22 days), weaned juvenile (45-60 days), and young adult pigs (>90 days) were fixed and stained with hematoxylin and eosin. Sections were examined under a fluorescent microscope to evaluate exocrine zymogen fluorescence intensity (ZFI) and under an electron microscope to evaluate exocrine zymogen granule density (ZGD). Results Exocrine content estimation showed significantly lower ZFI and ZGD in juvenile pig pancreases (1.5 ± 0.04 U/μm2, ZFI; 1.03 ± 0.07 × 103/100 μm2, ZGD) compared to young adult pigs (2.4 ± 0.05U/μm2, ZFI; 1.53 ± 0.08 × 103/100 μm2 ZGD). Islets in juvenile pig pancreases were on average smaller (105.2 ± 11.2 μm) than islets in young adult pigs (192 ± 7.7 μm), but their insulin content was comparable (80.9 ± 2.2% juvenile; 84.2 ± 0.3% young adult, P > 0.05). All data expressed as mean ± SEM. Conclusion Porcine islet xenotransplantation continues to make strides toward utilization in clinical trials of type 1 diabetes. Porcine donor age and weaning status influence the extent of exocrine maturation of the pancreas. Juvenile porcine pancreases may represent an alternative donor source for islet xenotransplantation as their exocrine component is relatively immature; this preserves islet viability during extended tissue culture following isolation.

Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.

Main Content
Current View