Skip to main content
eScholarship
Open Access Publications from the University of California

Rapid identification of emerging pathogens: Coronavirus

  • Author(s): Sampath, R
  • Hofstadler, SA
  • Blyn, LB
  • Eshoo, MW
  • Hall, TA
  • Massire, C
  • Levene, HM
  • Hannis, JC
  • Harrell, PM
  • Neuman, B
  • Buchmeier, MJ
  • Jiang, Y
  • Ranken, R
  • Drader, JJ
  • Samant, V
  • Griffey, RH
  • McNeil, JA
  • Crooke, ST
  • Ecker, DJ
  • et al.
Abstract

We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to identify organisms in a sample. We demonstrate this principle by using 14 isolates of 9 diverse Coronavirus spp., including the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). We show that this method could identify and distinguish between SARS and other known CoV, including the human CoV 229E and OC43, individually and in a mixture of all 3 human viruses. The sensitivity of detection, measured by using titered SARS-CoV spiked into human serum, was ≈1 PFU/mL. This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day.

Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.

Main Content
Current View