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Improving FRET dynamic range with bright green and red fluorescent proteins
- Lam, Amy J;
- St-Pierre, François;
- Gong, Yiyang;
- Marshall, Jesse D;
- Cranfill, Paula J;
- Baird, Michelle A;
- McKeown, Michael R;
- Wiedenmann, Jörg;
- Davidson, Michael W;
- Schnitzer, Mark J;
- Tsien, Roger Y;
- Lin, Michael Z
Published Web Location
https://doi.org/10.1038/nmeth.2171Abstract
Development of the bright green and red fluorescent proteins, Clover and mRuby2, creates a fluorescence resonance energy transfer (FRET) pair with the highest Förster radius among existing ratiometric FRET pairs. Substitution of this pair for current FRET pairs in several existing sensors reliably and substantially improves sensor performance.
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